Postnatal male germ-cell expression of cre recombinase in Tex101-iCre transgenic mice
نویسندگان
چکیده
منابع مشابه
Podocyte-specific expression of cre recombinase in transgenic mice.
We report a transgenic mouse line that expresses Cre recombinase exclusively in podocytes. Twenty- four transgenic founders were generated in which Cre recombinase was placed under the regulation of a 2.5-kb fragment of the human NPHS2 promoter. Previously, this fragment was shown to drive beta-galactosidase (beta-gal) expression exclusively in podocytes of transgenic mice. For analysis, founde...
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PURPOSE Gene inactivation with homologous recombination in mice is a widely used tool to study gene function. However, many proteins play essential roles in a number of tissues and germline gene inactivation often results in embryonic lethality. To overcome this limitation and to dissect the functions of essential genes beyond embryonic development, we generated mouse rod opsin promoter-control...
متن کاملExpression of an AQP2 Cre recombinase transgene in kidney and male reproductive system of transgenic mice.
A transgenic mouse approach was used to examine the mechanism of principal cell-specific expression of aquaporin-2 (AQP2) within the renal collecting duct. RT-PCR and immunocytochemistry revealed that murine AQP2 was expressed in principal cells in the renal collecting duct, epithelial cells of the vas deferens, and seminiferous tubules within testis. The vas deferens expression was confirmed i...
متن کاملCytokeratin 19 promoter directs the expression of Cre recombinase in various epithelia of transgenic mice
Cytokeratin 19 (K19) is expressed in various differentiated cells, including gastric, intestinal and bronchial epithelial cells, and liver duct cells. Here, we generated a transgenic mouse line, K19-Cre, in which the expression of Cre recombinase was controlled by the promoter of K19. To test the tissue distribution and excision activity of Cre recombinase, K19-Cre transgenic mice were bred wit...
متن کاملTie-1-directed expression of Cre recombinase in endothelial cells of embryoid bodies and transgenic mice.
Tissue-specific gene inactivation using the Cre-loxP system has become an important tool to unravel functions of genes when the conventional null mutation is lethal. We report here the generation of a transgenic mouse line expressing Cre recombinase in endothelial cells. In order to avoid the production and screening of multiple transgenic lines we used embryonic stem cell and embryoid body tec...
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ژورنال
عنوان ژورنال: genesis
سال: 2010
ISSN: 1526-954X
DOI: 10.1002/dvg.20675